Journal article
Influence of charge on hemocompatibility and immunoreactivity of polymeric nanoparticles
L Chen, JJ Glass, R De Rose, C Sperling, SJ Kent, ZH Houston, NL Fletcher, BE Rolfe, KJ Thurecht
ACS Applied Bio Materials | Published : 2018
Abstract
The benefits of nanomedicine may be restricted by hemocompatibility and immunoreactivity problems arising from administration of exogenous materials into the bloodstream. To understand how surface charge influences the interaction of polymeric nanoparticles with blood components, we synthesized three well-defined, chargevaried hyperbranched polymers (HBPs) of similar size and analyzed both hemocompatibility and immunoreactivity of these methacrylate-based HBPs ex vivo using primary human blood cell assays and image analyses following intravenous injection into mice. The results show that, regardless of charge, endotoxin-free HBPs had minimal effects on coagulation, platelet, complement, or T..
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Awarded by Australian National Fabrication Facility
Funding Acknowledgements
The authors acknowledge the Australian Research Council (LP150100703 (K.J.T.), DP140100951 (K.J.T.)) and National Health and Medical Research Council (APP1099321, APP1148582 (K.J.T.), APP1148582 (B.E.R.)) for funding. This work was performed in part at the Queensland node of the Australian National Fabrication Facility (ANFF), a company established under the National Collaborative Research Infrastructure Strategy to provide nano-and microfabrication facilities for Australia's researchers. This research was conducted and funded in part by the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology (CE140100036), the National Imaging Facility (NIF), and a grant from the University of Queensland Collaboration and Industry Engagement Fund. L.C. thanks the Chinese Scholarship Council for financial support.